User manual ZEISS UD 124

[. . . ] UD 124 Universal Rotary Stage Operating manual Knowledge of this manual is required for the operation of the instrument. Please therefore familiarize yourself with its contents and pay special regard to the sections dealing with the safe handling of the instrument. © Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted. Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model. Please contact: Carl Zeiss Mikroskopie D-07740 Jena Telefon: (03641) 64-1616 Fax: (03641) 64-3144 Internet: mikro@zeiss. de http://www. zeiss. de Subject to change B 40-017 e / 12. 96 Contents 1 Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 2 System overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 3 Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 4 Instructions for use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 4. 1 Adaptation of universal rotary stage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 4. 2 Preparation of universal rotary stage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 4. 2. 1 Positioning the sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 4. 2. 2 Imaging the sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 4. 2. 3 Centering the universal rotary stage to the microscope axis . . . . . . . . . . . . 8 4. 2. 4 Vertical adjustment of sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 4. 2. 5 Aligning the A2 and A4 axes to the eyepiece crosslines . . . . . . . . . . . . . . . . . . . . 8 4. 3 Application examples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 4. 3. 1 Orthoscopic work in linearly polarized light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 4. 3. 2 Conoscopic work in linearly polarized light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 4. 3. 3 Work in circularly polarized light. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 5 Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] When the correct height has been reached, lightly tighten the screw (Fig. 3/22), if necessary. 4. 2. 2 Imaging the sample Sharply image the sample and use the condenser to illuminate it (as specified in the microscope manual). Sharply image the narrowed luminous field diaphragm together with the sample, center and open it until the field of view is visible. 4. 2. 5 Aligning the A2 and A4 axes to the eyepiece crosslines Tilt the universal rotary stage about the A4 and A2 axes while observing the direction in which a dust particle below or above the object plane is moving. When the stage is tilted about the A4 axis, the particle must move parallel to the vertical line of the eyepiece crosslines. It it fails to do so, disengage the stage click stop mechanism and perform the necessary alignment by rotating the stage through the A5 axis. . Activate the stage click stop mechanism. When the stage is tilted about the A2 axis, the particle must move parallel to the horizontal (left-right), when tilted about the A4 axis, parallel to the vertical eyepiece crossline (topbottom). This is the standard setting. 4. 2. 3 Centering the universal rotary stage to the microscope axis The universal rotary stage of the Axiolab Pol has already been centered by the manufacturer. With the polarization microscopes Axioplan Pol, Axioplan 2 Pol, Axiophot Pol and Axiophot 2 Pol the universal rotary stage must be centered relative to the rotary axis of the microscope stage. 8 B 40 - 017 e / 12. 96 4 Instructions for use 4. 3 Application examples 4. 3. 1 Orthoscopic work in linearly polarized light Example 1: Measuring the angle 2 V of the optical axis of a biaxial crystal Center the selected grain in the crosslines. Switch in the 5x/0. 13 objective (standard use). Adjust the iris diaphragms of the 20x/0. 30 and 4. 3. 2 Conoscopic work in linearly polarized light The advantage of the conoscopic over the orthoscopic method is that the axial directions of monaxial or biaxial crystals can be identified directly in the interference image. 50x/0. 60) LD (long distance) objective and a Bertrand lens permitting observation of the rear focal plane of the objective are required. Example 2: Measuring the angle 2 V of the optical axis of a biaxial crystal (conoscopically) Proceed as described in example 1 (steps 1 to 50x/0. 60 objectives and the aperture diaphragm of the condenser (Fig. 3/12) to increase the contrast of the Axiolab Pol (same procedure for Axioplan-microscopes). Rotate through the A1 axis to dark position. Nor- 10). Switch in the 50x/0. 60 objective. Switch in the Bertrand lens (focus Axioplan mi- mally this will result in brightening up. Tilt about the A2 axis to dark position. Tilt about the A4 axis in opposite direction until it croscopes). Use the graduated circle and the vernier brightens up. Rotate through the A1 axis to extinction. If the grain remains dark when the stage is tilted about the A4 axis, the plane of the optical axis lies parallel to the microscope's symmetry plane (if not, repeat the previously described steps). Clamp the A2 axis (Fig. For Axioplan microscopes, release the clamp- (Fig. 3/19) to measure the optical axis by tilting about A4. The axial directions can be identified from the curved, dark , hyperbola branches. The exit point of the crystal's optical axis lies in the vertex of the hyperbola. [. . . ] and rinse off the immersion fluid with clean water, or dab it off using wet cotton wool. Distilled water is recommended for glycerin and light gasolin for immersion oil. Use an optical cleaning cloth or leather for the actual cleaning process. way. Carefully separate these components sideways The mechanical parts are cleaned in the same Caution! [. . . ]